Promoting the program's debut, a newspaper announcement noted that the United States was then at war and said, "This Is Your FBI will show graphically exactly how enemies of the United States have, and are, operating, and how FBI agents and American citizens are cooperating to circumvent their activities." It added that crime was not glamorous but "a dirty stab at every citizen and his country".
Jerry Devine produced the series. Van Cleave was the initial musical director, leading an 18-man orchestra. Stacy Harris (July 26, 1918 – March 13, 1973) was an American actor with hundreds of film and television appearances. His name is sometimes found misspelled Stacey Harris.
Harris was known for his role as agent Jim Taylor on this series. In 1946, Jerry Devine, that program's producer-director, told newspaper columnist Jack O'Brian: "Stacy has just the sort of voice I need for the quiet authority of the special agent on my show. On top of that, he's a good actor, and it's a combination on radio which can't be beat."
His other roles in radio programs included Batman in The Adventures of Superman, and Ted Blades in The Strange Romance of Evelyn Winters. He was also a member of the casts of Confession, Dragnet, Pepper Young's Family, Destiny's Trails, and Frontier Gentleman.